In an attempt to detect protein expression profile in a cell, Western blot techmque is employed. Expression of two new proteins is to be followed by probing with respective high […]
Tag: Northern blotting
Tag: Northern blotting
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Which Technique is the Exception for Protein-Protein Interactions
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- March 4, 2025
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Which technique is the exception for the evaluation of protein-protein interaction: A. Far-Western blotting B. Chromatin immune precipitation C. Yeast-two hybrid system D. Co-immuno precipitation Which Technique is the Exception […]
Zoo Blotting: Detecting Conserved DNA Sequences Across Species
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- March 3, 2025
- 61 Comments
Zoo Blotting: Detecting Conserved DNA Sequences Across Species A zoo blot helps to detect DNA sequences that A. Are mutating at a fast rate. B. Are conserved between species. C. […]
Detecting Viral Insertions in the Genome: The Role of Southern Blotting
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- March 3, 2025
- 82 Comments
Detecting Viral Insertions in the Genome: The Role of Southern Blotting The virus inserted in genome can be recognized by A. FISH B. Northern blot C. Microarray D. Southern blot […]
Southern Blotting: A Powerful Technique for DNA Fragment Detection
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- March 3, 2025
- 80 Comments
Southern Blotting: A Powerful Technique for DNA Fragment Detection The Southern blotting technique is used for the detection of: A. RNA fragmene on membranes by specific radioactive antibodies B. DNA […]
Detecting Alpha-Amylase Gene Polymorphism: The Role of Southern Blotting
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- March 3, 2025
- 68 Comments
Detecting Alpha-Amylase Gene Polymorphism: The Role of Southern Blotting Alpha-amylase gene polymorphism is determined by which technique? A. Southern blot B. Slot blot C. Dot blot D. Northern blot Which […]
Southern Blotting: The Best Technique for DNA Detection
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- March 3, 2025
- 83 Comments
Southern Blotting: The Best Technique for DNA Detection Which blotting technique is used for the detection of DNA that has been separated from a mixture of DNA restriction fragments by […]
Key Component Not Used in Western Blotting: Agarose Gel Electrophoresis
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- March 3, 2025
- 79 Comments
Key Component Not Used in Western Blotting: Agarose Gel Electrophoresis Which one of the following is not used during Western blotting? A. Secondary antibody with a conjugated detection system B. […]
Key Differences Between Southern and Northern Hybridizations
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- March 3, 2025
- 67 Comments
Key Differences Between Southern and Northern Hybridizations What is the difference between Southern and Northern hybridizations ? A. Southem blots hybridize a DNA probe to a digested DNA sample but […]
Why NaOH Hydrolyzes RNA in Northern Blot
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- March 3, 2025
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Why NaOH Hydrolyzes RNA in Northern Blot: The Role of the 2′-Hydroxyl Group The procedure of Southern blotting involves treatment of the solid support (nitrocellulose) containing the DNA with NaOH […]