csir pyq prokaryotic Transcription Archives - CSIR NET LIFE SCIENCE COACHING | NTA NET LIFE SCIENCE

6.An antibiotic that resembles the 3' end of a charged tRNA molecule is: (1) Streptomycin(2) Sporsomycin (3) Puromycin (4) Tetracycline

CSIR NET Life Science Previous Year Questions and Solution on Molecular Biology

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June 13, 2025
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6.An antibiotic that resembles the 3′ end of a charged tRNA molecule is: (1) Streptomycin(2) Sporsomycin (3) Puromycin (4) Tetracycline

1. Chloramphenicol is a "broad-spectrum" antibiotic which inhibits protein synthesis in prokaryotes. Given below are a few statements regarding the mode of action of chloramphenicol. A. Chloramphenicol inhibits the peptidyl-transferase activity of ribosomes. B. Chloramphenicol can be used to treat moderate to severe infections, because mitochondrial ribosomes are not sensitive to chloramphenicol. C. Chloramphenicol binds to one of the domains of 23S rRNA D. Chloramphenicol competes for binding with the E- sitetRNA Which of the following options describes correctly the mechanism of action of chloramphenicol? (1) B and D only (2) A and C only (3) A, C and D (4) B, C and D

CSIR NET Life Science Previous Year Questions and Solution on Molecular Biology

Chloramphenicol: Mechanism of Action, Ribosomal Binding, and Clinical Implications

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June 13, 2025
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1. Chloramphenicol is a “broad-spectrum” antibiotic which inhibits protein synthesis in prokaryotes. Given below are a few statements regarding the mode of action of chloramphenicol. A. Chloramphenicol inhibits the peptidyl-transferase […]

CSIR NET Life Science Previous Year Questions and Solution on Molecular Biology

Small Nucleolar RNAs and rRNA Modification

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May 31, 2025
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14. Small nucleolar RNAs used to process and chemically modify rRNAs are called (1) ScaRNAs (2) SiRNAs (3) SnoRNA (4) SnRNAs The correct answer is (3) SnoRNA. Small Nucleolar RNAs and […]

The following statements are related to transcription in bacteria and eukaryotes. A. During concurrent promoter sequence recognition and melting, melting commences with base flipping where two bases are flipped out into pockets of the primary sigma factor B. Binding of a-amanitin to RNA polymerase II permits entry of nucleotides into RNA pol II active site and synthesis of RNA, but prevents translocation. C.RNA polymerase I can use upstream promoters with 3 consensus sequences, as well as internal promoters having a bipartite structure D. FACT is associated with RNA polymerase during transcriptional elongation and helps displace histone octamers during transcription Which of the following combinations of statements represents all correct statements? (1) A, B and C (2) A, B and D (3) B, C and D (4) B and C only

CSIR NET Life Science Previous Year Questions and Solution on Molecular Biology

Transcription in Bacteria and Eukaryotes | RNA polymerase II | Bipartite structure

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May 31, 2025
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The following statements are related to transcription in bacteria and eukaryotes. A. During concurrent promoter sequence recognition and melting, melting commences with base flipping where two bases are flipped out […]

Functional tRNAs are produced even when their genes lack the -CCA end because the -CCA end is added during tRNA maturation

CSIR NET Life Science Previous Year Questions and Solution on Molecular Biology

Why Do Functional tRNAs Exist Even When Their Genes Lack the -CCA End

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May 29, 2025
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tRNA genes of a Gram positive bacteria were sequenced. While some of the genes possessed sequence corresponding to the -CCA end of the tRNA, others did not. Interestingly, even the […]

All of the following statements about bacterial transcription termination are true EXCEPT (1) some terminator sequences require Rho protein for termination. (2) inverted repeat and ‘T’ rich non-template strand define intrinsic terminators. (3) Rho-dependent terminators may possess inverted repeat elements. Rho (4) Nus A is necessary for intrinsic transcription termination.

CSIR NET Life Science Previous Year Questions and Solution on Molecular Biology

Bacterial Transcription Termination Role of NusA

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May 28, 2025
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All of the following statements about bacterial transcription termination are true EXCEPT (1) some terminator sequences require Rho protein for termination. (2) inverted repeat and ‘T’ rich non-template strand define […]

15. Rho factor is involved in termination of transcription in prokaryotes. Genetic manipulations indicate that Rho-dependent termination requires the presence of a specific recognition sequence on the newly synthesized RNA upstream of the termination site. The recognition sequence must be on the necent RNA rather then the DNA, as demonstrated by Rho’s inability to terminate transcription in the presence of pancreatic RNAse. The essential features of this termination site have not been fully elucidated. Construction of synthetic termination sites indicates that it consists of 80 to 100 nts that lack a stable secondary structure and contain multiple regions that are rich in C and poor in G. Which of the following is/ are suggested by the above observation? (1) Rho factor attaches to nascent RNA at its recognition sequence and then migrates along the RNA in the 5'-->3. direction until it encounters an RNAP paused at the termination site and disrupts the transcriptional complex by acting as an RNA-DNA helicase. (2) Rho unwinds the RNA-DNA duplex forming the transcription bubble, thereby releasing the RNA transcript. (3) Rho factor attaches to the RNA at its recognition sequence while RNA is in the RNA-DNA hybrid condition. (4) There may be other factors and hence Rho factor does not need to unwind the RNA-DNA hybrid to release the transcript.

CSIR NET Life Science Previous Year Questions and Solution on Molecular Biology

Rho-Dependent Termination | hexameric ATP-dependent RNA helicase Rho

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May 28, 2025
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15. Rho factor is involved in termination of transcription in prokaryotes. Genetic manipulations indicate that Rho-dependent termination requires the presence of a specific recognition sequence on the newly synthesized RNA […]

14. Actinomycin D inhibits the process of transcription in both prokaryotic and eukaryotic organisms. The following statements are made about actinomycin D-mediated inhibition of transcription: (1) Actinomycin D inhibits transcription from a double stranded DNA template by either E. coil or yeast RNA polymerases. (2) Actinomycin D inhibits transcription from a single stranded RNA template by eukaryotic viral RNA (3) Actinomycin D inhibits transcription from single stranded Φ x 174 DNA template by E. coli RNA polymerase, immediately after viral DNA entry. (4) Actinomycin D inhibits transcription from double stranded RNA template by eukaryotic RNA polymerase II. Which of the combinations of the above statements is a true representation mediated inhibition ? of the mechanism of actinomycin D (1) A only (2) A, B and D (3) A, B and C (4) A and D only

CSIR NET Life Science Previous Year Questions and Solution on Molecular Biology

Actinomycin D inhibits transcription in both prokaryotic and eukaryotic

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May 28, 2025
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14. Actinomycin D inhibits the process of transcription in both prokaryotic and eukaryotic organisms. The following statements are made about actinomycin D-mediated inhibition of transcription: (1) Actinomycin D inhibits transcription […]

13. Which one of the following statements is NOT a correct feature of Escherichia coli RNA polymerase? (1) Presence of the σ subunit along with α2ββ’ω core RNA polymerase is required for its promoter specific binding. (2) Presence of the σ subunit along with α2ββ’ω core RNA polymerase is not necessary for the core RNA polymerase α2ββ’ω to bind to the DNA template. (3) Mutations in β subunit in its rifampicin resistance defining region (RRDR) confer rifampicin resistance phenotype. (4) Mutations in σ subunit in its rifampicin resistance defining region (RRDR) confer rifampicin resistance phenotype.

CSIR NET Life Science Previous Year Questions and Solution on Molecular Biology

Escherichia coli RNA polymerase α2ββ’ω Components rifampicin

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May 28, 2025
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13. Which one of the following statements is NOT a correct feature of Escherichia coli RNA polymerase? (1) Presence of the σ subunit along with α2ββ’ω core RNA polymerase is […]

12. One of the mechanism is used by bacteria for adaptation to changed environment is altering transcription of their genes. In this regard, which one of the following responses is NOT found in bacteria? (1) A gene with two different promoters for expression in different conditions (2) use of different sigma factors for transcription of genes (3) Expression of alternate α,β and β’ subunits (4) Expression of anti-sigma factors

CSIR NET Life Science Previous Year Questions and Solution on Molecular Biology

How Bacteria Adapt to Environmental Changes: Mechanisms of Gene Regulation Explained”

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May 28, 2025
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How Bacteria Adapt to Environmental Changes: Mechanisms of Gene Regulation Explained 12. One of the mechanism is used by bacteria for adaptation to changed environment is altering transcription of […]

Tag: csir pyq prokaryotic Transcription

Chloramphenicol: Mechanism of Action, Ribosomal Binding, and Clinical Implications

Small Nucleolar RNAs and rRNA Modification

Transcription in Bacteria and Eukaryotes | RNA polymerase II | Bipartite structure

Why Do Functional tRNAs Exist Even When Their Genes Lack the -CCA End

Bacterial Transcription Termination Role of NusA

Rho-Dependent Termination | hexameric ATP-dependent RNA helicase Rho

Actinomycin D inhibits transcription in both prokaryotic and eukaryotic

Escherichia coli RNA polymerase α2ββ’ω Components rifampicin

How Bacteria Adapt to Environmental Changes: Mechanisms of Gene Regulation Explained”

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