In electrophoretic separation of proteins in an agarose gel, electroendosmotic flow occurs. This is a buffer flow that may disturb protein migration in the gel. Which of the following alternatives […]
Tag: Isoelectric focusing (IEF)
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CSIR NET Previous Year Questions Gel Electrophoresis
What is an issue with using 2D-PAGE
What is an issue with using 2D-PAGE? a. Hydrophobic proteins may not run as expected due to the hydrophobic surfaces. b. Some proteins may not migrate through polyacrylamide and therefore […]
CSIR NET Previous Year Questions Gel Electrophoresis
Isoelectric focusing separation of protein-on positive and negative
What is the basis of isoelectric focusing separation of protein. (a) Relative content of positively charged residues only (b) Relative content of negatively charged residues only (c) Size (d) Relative […]
CSIR NET Previous Year Questions Gel Electrophoresis
Technique that can be used to analyze proteins-isoelectric focusing?
The correct statement for a technique that can be used to analyze proteins. Which of the following is true of isoelectric focusing? (a) It is commonly used to quantitate IgA […]
