Tag: dbt jrf 2018

188. A culture can grow independently in two carbon sources, glucose and hexadecane. Identify which one of the following statements is TRUE? 1. The biomass growth yield is more in […]

187. Consider the scale up of a fermentation from a 10 L to 10,000 L while maintaining geometric similarity. Agitation speed was maintained at 500 rpm in the 10 L […]

186. A recombinant protein is produced in Escherichia coli by two stage continuous cultivation at steady state. Upon induction of the culture in stage II, the specific growth rate of […]

185. In a fed batch cultivation, a specific growth rate of 0.2/h needs to be maintained. At the start of fedbatch cultivation, 200 mL/h of media is fed to 1000 […]

183. We wish to produce a metabolite ‘X’ whose biosynthetic pathway is feedback inhibited in the wild type strain. A mutation, which leads to overproduction of X, is discovered in […]

182. In a continuous crystallizer, 100 g of a saturated solution of sugar at 85% (w/w) enters the crystallizer and leaves the crystallizer at 70% (w/w). The weight of input […]

181. In a CSTR, first order reaction takes place converting A to B. If at a dilution rate (D)= 0.5 h-1 , 50% of A gets converted to B, then […]

180. In a plug flow bioreactor running at steady state, 12.5% cells are recycled back to the inlet. If the cells grow at μmax=1 h-1 throughout the length of the […]

179. A high cell density fermentation produces recombinant protein product which is 20% of the total cellular protein. The final cell density is 80 OD (1 OD = 0.4g of […]

178. An enzyme follows Michaelis-Menten kinetics with the following parameters: Vmax= 5 mM/s and Km= 2.5 mM. The reaction velocity would be: 1. 2.5 mM/s at all substrate concentration 2. […]