Serum protein is separated in 1% agarose gel, 25minutes at 100 volts, stained with Ponseau S protein stain. The separation of the protein is not optimal. What would you do […]
Tag: 2D-PAGE
No posts found.
CSIR NET Previous Year Questions Gel Electrophoresis
Electroendosmotic flow disturb protein migration in the gel
In electrophoretic separation of proteins in an agarose gel, electroendosmotic flow occurs. This is a buffer flow that may disturb protein migration in the gel. Which of the following alternatives […]
CSIR NET Previous Year Questions Gel Electrophoresis
Total charge of a protein in native gel is determined by the pH
In an electrophoresis system, using a native gel (agarose) proteins are separated due to difference in total charge. What decides the charge of protein ? (a) Voltage (b) Buffer’s pH […]
CSIR NET Previous Year Questions Gel Electrophoresis
2D gel electrophoresis Isoelectric Focusing (IEF) and SDS-PAGE
Which of the following statement about 2D gel electrophoresis is correct? (a) SDS gel electrophoresis and isoelectric focusing both separate native proteins (b) SDS gel electrophoresis and isoelectric focusing both […]
CSIR NET Previous Year Questions Gel Electrophoresis
Isoelectric Focusing-The separation of proteins is only based on charge
Which of the following statement about isoelectric focusing is correct (a) Proteins separated by isoelectric focusing cannot be tested for biological activity (b) Proteins separated by it can be tested […]
CSIR NET Previous Year Questions Gel Electrophoresis
Two dimensional gel electrophoresis is a technique for separating proteins
Two dimensional gel electrophoresis is a technique for separating proteins (A) Based on charge (B) Based on mass (C) Based on both charge and mass (D) Based on its pI […]
CSIR NET Previous Year Questions Gel Electrophoresis
What is an issue with using 2D-PAGE
What is an issue with using 2D-PAGE? a. Hydrophobic proteins may not run as expected due to the hydrophobic surfaces. b. Some proteins may not migrate through polyacrylamide and therefore […]
