Applications of PCR in Genetic Research
Which of the following is an application for PCR?
a. Site-directed mutagenesis
b. Creation of insertions, deletions, and fusions of different gene segments
c. Amplification of specific segments of DNA
d. For cloning into vector
Polymerase Chain Reaction (PCR) is one of the most widely used techniques in molecular biology and genetic research. It allows scientists to amplify specific DNA sequences, making it a powerful tool for various applications in genetic engineering, diagnostics, and biotechnology.
Correct Answer:
The correct answer is (c) Amplification of specific segments of DNA.
Why Amplification is the Primary Use of PCR:
PCR enables the rapid amplification of specific DNA segments, even from minute amounts of starting material. Through repeated cycles of denaturation, annealing, and extension, PCR can generate millions of copies of a targeted DNA region.
Other Applications of PCR:
While amplification is the core purpose of PCR, it is also used in:
- (a) Site-directed mutagenesis – PCR can introduce specific mutations in DNA sequences to study gene function.
- (b) Creation of insertions, deletions, and fusions of different gene segments – Modified PCR methods enable gene editing and structural alterations in DNA.
- (d) For cloning into vector – Amplified PCR products can be inserted into vectors for gene expression studies or recombinant DNA technology.
Conclusion:
PCR remains a cornerstone of genetic research due to its ability to amplify DNA with high specificity and accuracy. Its applications extend to diagnostics, forensic science, and gene editing, making it an indispensable tool in modern molecular biology.
79 Comments
Rohit Meena
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Akshay mahawar
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Abhilasha
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Khushi Pareek
August 24, 2025Specific DNA amplification
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August 24, 2025C
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August 24, 2025Amplification of specific segments of DNA.
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August 24, 2025Amplification of specific DNA segments
Mansukh Kapoor
August 24, 2025The correct answer is option 3rd
Amplification of specific segment of DNA
Avni
August 24, 2025The correct answer is (c) Amplification of specific segments of DNA.
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Khushi Agarwal
August 24, 2025Option c is correct
Amplification of specific segments of DNA.
Anurag Giri
August 24, 2025Ans 3
PCR enables the rapid amplification of specific DNA segments, even from minute amounts of starting material. Through repeated cycles of denaturation, annealing, and extension
Dharmpal Swami
August 24, 2025PCR is used for amplification of specific DNA sequences
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August 24, 2025For amplification of specific DNA sequence
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Priyanka Choudhary
August 24, 2025C will be right answer,
Amplification of specific segments of DNA.
Karishma
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Priya dhakad
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August 24, 2025Answer c
Amplification of specific segments of DNA.
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Bhawna Choudhary
August 24, 2025Correct answer is C amplification of specific sequence of DNa
Priyanka Verma
August 24, 2025Option c is correct- Amplification of specific segments of DNA.
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August 24, 2025Option d amplification of specific segments of dna
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Aman Choudhary
August 25, 2025Option C is correct
Amplification of specific segment DNA
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Vanshika Sharma
August 26, 2025Ans is 3
Minal Sethi
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Shivani
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Rishita
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Alec
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Mitali saini
August 28, 2025The correct answer is (c) Amplification of specific segments of DNA.
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August 28, 2025Amplification of specific segment of dna
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August 28, 2025done
Nilofar Khan
August 28, 2025Correct answer is (c) Amplification of specific segments of DNA
Kajal
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August 28, 2025Option C is correct
Khushi Vaishnav
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Priya dhakad
August 29, 2025Amplification of specific segments of DNA.
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Parul Yadav
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August 30, 2025Amplification of specific segment of dna
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Kajal
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September 3, 2025Amplification of specific segments of DNA
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September 8, 2025Amplification of specific segments of DNA.
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September 9, 2025Amplification of specific segments of DNA.