image                                                                                                                                                                                                                                                                                                      image

RASA LSI with Central Dogma organizing Industrial Short term training
This summer get expertise in Molecular Biology Techniques and Bio-informatics 
Apply Here
The primary goal of this program in this field is to educate students for jobs in biotechnology, pharmaceuticals, and related industry sectors. The curriculum is extremely flexible, allowing for dual majors with several other disciplines. Revolutions in biotechnology and information technology are changing the world. This program will make you understand basics of Bio-Informatic application in molecular biology with extensive hands on molecular laboratory work. 
Hands on training in molecular biology with a concentration in bioinformatics may attract those desiring an M.S. degree before proceeding to professional study in medicine or a doctoral program. 
Why join us?
Through appropriate elective selection, students planning careers as molecular biologists with a computational background or as fully trained computer scientists with knowledge of biological sciences can adapt the program to their needs. 
There are extensive opportunities to pursue undergraduate research in faculty laboratories. The bioinformatics and molecular biology program also serves as an excellent premedical curriculum.
Eligibility: Students- Graduate, Post Graduate, Professionals, PhD, Post Doc., Fresher. 
Background: Life sciences, Biotechnology, Pharma, BioChemistry, Bio IT, Life Science  
Software.
Topics to be covered
Fundamental Techniques in Genomics and Proteomics
Week 1: Bioinformatics
1. Introduction to bioinformatics and Databases
· Definition of Bioinformatics, databases
· Introduction to NCBI, EMBL and DDBJ, Protein databases (primary, composite and secondary)
· Generation of large scale molecular biology data
· Applications of Bioinformatics – Sequencing, NMR Spectroscopy, X – ray, Gel electrophoresis)
2. Sequence alignment 
· Introduction to dynamic programming and sequence alignment
· Pairwise sequence alignment (BLAST and FASTA)
· Multiple sequence alignment (ClustalW)
· Concept of homology, similarity, identity, orthologues and paralogues
3. Primer designing – Primer BLAST
Designing primers using Primer BLAST for PCR
Week 2: Molecular Biology
1. Plasmid DNA (vector) isolation from bacterial culture, Agarose gel electrophoresis (Casting the gel, Loading)
2. Plasmid mapping using multiple restriction enzymes, Agarose gel electrophoresis
3. Theory on PCR and Primer design for PCR, Gene amplification by Polymerase chain reaction, Agarose gel electrophoresis to check gene amplification
4. Competent cell preparation (Bacterial host), Transformation of competent cells with expression vector carrying specific gene, Plating the transformants on the LB agar +Amp plates and overnight incubation
Week 3: Bioinformatics
1. Restriction enzyme mapping
Mapping of restriction enzyme and enzyme cutting using Restriction Mapper tool
2. Sequence Extractor
Generating primer map and enzyme map using Sequence extractor
3. Insilico vector design
Designing vector using insilico techniques
4. Protein Databases, structure cleaning and downloading , and Protein visualization
Introduction to protein databases, cleaning and downloading of protein structure
Protein visualization using Discovery Studio and SPDBV
Week 4: Molecular techniques
1. Colony PCR analysis of transformants
2. Growth curve of bacterial host and bacterial host carrying plasmid (Spectrophotometric analysis)
3. Casting a polyacryalamide gel and 
4. Protein sample preparation and gel loading, Electrophoresis and 
Staining the gel with Coomasie Blue for visualization of proteins.
Apply Here
Batches and Deadline for Registrations:
· Batch 1: 1st March 2017 Dead Line : 25th Feb 2017
· Batch 2: 27th March 2017 Dead Line: 5th March 2017
· Batch 3: 20th April 2017 Dead Line: 5th April 2017
· Duration: 4 weeks
TERM AND CONDITIONS:
· Fees are likely to change every two months.
· Fees are non transferrable & non refundable in any case.
· Accommodation assistance for out- station candidates.
www.rasalsi.com
Please feel free to contact us at any time:
RASA Life Science Informatics
4th floor.46/10 Shakuntal Building, 
Law college Road, Near Pastry corner, Nal stop,

Erandwane, Pune -411004 
Phone No. 020-65600408
/ 8411927535/ 8554874709/ 7875793891.
Website: www.rasalsi.com 
http://centraldogma.co.in/

| Let's talk Acdemy"/>
  • +919785333312/14
  • info@letstalkacademy.com

Announcement

This summer get expertise in Molecular Biology techniques and Bio-informatics

2017-02-22

image                                                                                                                                                                                                                                                                                                      image

RASA LSI with Central Dogma organizing Industrial Short term training
This summer get expertise in Molecular Biology Techniques and Bio-informatics 
Apply Here
The primary goal of this program in this field is to educate students for jobs in biotechnology, pharmaceuticals, and related industry sectors. The curriculum is extremely flexible, allowing for dual majors with several other disciplines. Revolutions in biotechnology and information technology are changing the world. This program will make you understand basics of Bio-Informatic application in molecular biology with extensive hands on molecular laboratory work. 
Hands on training in molecular biology with a concentration in bioinformatics may attract those desiring an M.S. degree before proceeding to professional study in medicine or a doctoral program. 
Why join us?
Through appropriate elective selection, students planning careers as molecular biologists with a computational background or as fully trained computer scientists with knowledge of biological sciences can adapt the program to their needs. 
There are extensive opportunities to pursue undergraduate research in faculty laboratories. The bioinformatics and molecular biology program also serves as an excellent premedical curriculum.
Eligibility: Students- Graduate, Post Graduate, Professionals, PhD, Post Doc., Fresher. 
Background: Life sciences, Biotechnology, Pharma, BioChemistry, Bio IT, Life Science  
Software.
Topics to be covered
Fundamental Techniques in Genomics and Proteomics
Week 1: Bioinformatics
1. Introduction to bioinformatics and Databases
· Definition of Bioinformatics, databases
· Introduction to NCBI, EMBL and DDBJ, Protein databases (primary, composite and secondary)
· Generation of large scale molecular biology data
· Applications of Bioinformatics – Sequencing, NMR Spectroscopy, X – ray, Gel electrophoresis)
2. Sequence alignment 
· Introduction to dynamic programming and sequence alignment
· Pairwise sequence alignment (BLAST and FASTA)
· Multiple sequence alignment (ClustalW)
· Concept of homology, similarity, identity, orthologues and paralogues
3. Primer designing – Primer BLAST
Designing primers using Primer BLAST for PCR
Week 2: Molecular Biology
1. Plasmid DNA (vector) isolation from bacterial culture, Agarose gel electrophoresis (Casting the gel, Loading)
2. Plasmid mapping using multiple restriction enzymes, Agarose gel electrophoresis
3. Theory on PCR and Primer design for PCR, Gene amplification by Polymerase chain reaction, Agarose gel electrophoresis to check gene amplification
4. Competent cell preparation (Bacterial host), Transformation of competent cells with expression vector carrying specific gene, Plating the transformants on the LB agar +Amp plates and overnight incubation
Week 3: Bioinformatics
1. Restriction enzyme mapping
Mapping of restriction enzyme and enzyme cutting using Restriction Mapper tool
2. Sequence Extractor
Generating primer map and enzyme map using Sequence extractor
3. Insilico vector design
Designing vector using insilico techniques
4. Protein Databases, structure cleaning and downloading , and Protein visualization
Introduction to protein databases, cleaning and downloading of protein structure
Protein visualization using Discovery Studio and SPDBV
Week 4: Molecular techniques
1. Colony PCR analysis of transformants
2. Growth curve of bacterial host and bacterial host carrying plasmid (Spectrophotometric analysis)
3. Casting a polyacryalamide gel and 
4. Protein sample preparation and gel loading, Electrophoresis and 
Staining the gel with Coomasie Blue for visualization of proteins.
Apply Here
Batches and Deadline for Registrations:
· Batch 1: 1st March 2017 Dead Line : 25th Feb 2017
· Batch 2: 27th March 2017 Dead Line: 5th March 2017
· Batch 3: 20th April 2017 Dead Line: 5th April 2017
· Duration: 4 weeks
TERM AND CONDITIONS:
· Fees are likely to change every two months.
· Fees are non transferrable & non refundable in any case.
· Accommodation assistance for out- station candidates.
www.rasalsi.com
Please feel free to contact us at any time:
RASA Life Science Informatics
4th floor.46/10 Shakuntal Building, 
Law college Road, Near Pastry corner, Nal stop,

Erandwane, Pune -411004 
Phone No. 020-65600408
/ 8411927535/ 8554874709/ 7875793891.
Website: www.rasalsi.com 
http://centraldogma.co.in/

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